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Hygromycin B is an aminoglycoside produced by Streptomyces hygroscopicus. It is used as an anthelmintic against swine infections by large roundworms. Backbone · Vector backbone. pcDNA4-TO · Backbone manufacturer. Invitrogen · Backbone size (bp) · Modifications to backbone · Vector type · Selectable markers. Hygromycin B antibiotic ensures effective positive selection of cells expressing the hygromycin resistance. (hph) gene. In mammalian cells, the recommended.

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Hygromycin and Kanamycin resistant. Marker: kanamycin, hygromycin minigene constructed using Multisite Gateway Technology (Invitrogen) by fusing the. Mammalian expression vector with the CMV promoter. The MCS is in the forward (+) orientation. Sequence Author: Thermo Fisher (Invitrogen). Open in SnapGene Try. Biochemical [] Hygromycin B (50 mg/mL) by Invitrogen Antibodies.

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purchased from Invitrogen, and more detailed information can be found in their will become hygromycin B resistant, as this marker is also incorporated. Hygromycin B acts by binding to the 80S subunit of the bacterial ribosome and inhibiting protein synthesis. Gibco™ Hygromycin B is used as a bacterial selection. Schneider's Drosophila Medium is available separately from Invitrogen expression vector, we generally use µg/ml hygromycin-B. For S2 cells stably.